Aug 11, 2015


There’s a lot that can go wrong with high-performance liquid chromatography (HPLC). The pressure of the system can get crazy, the lines can get blocked, and the columns don’t last forever.  While we can’t solve all of your HPLC problems, we will periodically post some suggestions to help you maintain your HPLC to the best of your ability.  To receive more tips and tricks be sure to follow us on twitter and linkedin.

How can you reduce LC problems?

You can reduce many LC problems by eliminating the most common sources of flow blockage. One of the many ways you can do this is by constantly filtering. Many particles leading to blockage can come from sources located both outside and inside the LC system such as; solvent, buffer, microbial growth in solvent reservoirs, the sample, and wear of the LC components (piston seals, autosampler valve, etc.). Much of the debris will either be captured on the column frit or in-line filter. In order to ensure that flow blockage does not happen preventative maintenance is key!

If you are interested in keeping your HPLC in good health, and would like more information about our services contracts, please call us at 860.691.2213 or visit our service page.

Check back next week for Six Steps to Clean or Replace the Solvent Inlet Filters as part of ALT’s Tips and Tricks.