Sep 09, 2015

TIPS AND TRICKS: OVERVIEW OF DEGASSER MAINTENANCE

There’s a lot that can go wrong with high-performance liquid chromatography (HPLC). The pressure of the system can get crazy, the lines can get blocked, and the columns don’t last forever.  While we can’t solve all of your HPLC problems, we will periodically post some suggestions to help you maintain your HPLC to the best of your ability.

Overview of Major Autosampler Maintenance:

In order to maintain the major autosampler in your HPLC you must replace the injection valve and rotor seal as well as the needle and the needle seat if applicable. After this is done, you should check and replace the metering unit seal. The transport rods and waste tubing should then both be cleaned and the leak sensor should be checked. Lastly, a pressure test should be performed.  This can be covered by an ALT maintenance contract!

How do you take care of your Detector Flow Cells?

It’s quite easy to take care of your HPLC’s Detector Flow Cells, to do so, avoid the use of alkaline solutions with pH > 9.5 which can attack quartz and impair the optical performance of your HPLC. Try to prevent the crystallization of buffers or salts which will lead to a blockage and then damage your HPLC. It is important to not leave 100% water standing in the flow cell as aqueous solvents can allow algae growth. When leaving the LC idle, it is a must to pump a mobile phase with at least 5-10% organic solvent.

How to maintain the Data System:

In order to maintain the data system on your HPLC you must first delete temporary files using the Disk Cleanup utility as well as check to scan and repair any errors that may be on the disk. Next, you should use virus detection software to defragment the hard drive and have a backup strategy for data, methods, sequences, custom UV-libraries, custom report templates, etc.

If you are interested in service maintenance and or contracts please contact us.