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Incubator Maintenance Checklist

March 15, 2024

Incubators are an essential part of many scientific laboratories, widely used for cell culture, microbiology, and molecular biology. The purpose of an incubator is to maintain an optimal environment for the growth and development of cells. However, to ensure the accuracy and validity of the experiments conducted, regular maintenance is necessary. This blog post will provide you with a comprehensive checklist for daily, weekly, monthly, and semi-annual incubator maintenance.  

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Daily Maintenance

On a daily basis, it is crucial to check for any spills inside the incubator. If any are found, clean them up immediately using a 70% isopropanol solution. This helps prevent contamination and maintains the cleanliness of the incubator. In addition, it is also essential to keep the water tray filled. Refill the water tray as needed to maintain the required humidity level inside the incubator.

Weekly Maintenance

Each week, take a moment to empty the water tray, clean it thoroughly, and refill it. This task helps to prevent the growth and spread of any potential contaminants. After this, you should verify and record the temperature, humidity, and CO₂ levels. These parameters are important for the optimal growth of cultures and should be monitored closely. 

Monthly Maintenance

A more thorough clean should be conducted on a monthly basis. Empty the incubator completely and wipe down all interior surfaces with a 70% isopropanol solution. Pay special attention to external surfaces that are frequently touched, such as door handles. Dust should be removed from the top and back of the unit to prevent any blockages or overheating. Additionally, inspect and clean the door gaskets. These tasks help to maintain a sterile environment and aid in the longevity of the incubator.

Every 6-12 Months

Semi-annual maintenance is equally as important as daily, weekly, and monthly maintenance. Every 6 to 12 months, calibrate the temperature, humidity, and CO₂ sensors to ensure they are providing accurate readings. Replace the HEPA filters, as these filters help to maintain a clean incubator environment by filtering out any potential contaminants. Lastly, schedule a routine maintenance visit from a professional. They can provide a more thorough check of the incubator and address any potential issues that may not be visible to the untrained eye.

Maintaining an incubator can seem like a daunting task, but with a systematic approach and routine checks, it can become a simple part of your laboratory practice. This checklist serves as a guide to keep your incubator in top condition, ensuring the success of your experiments and the longevity of your equipment. For more information, visit

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