AB Sciex API 3200 LC/MS/MS With Shimadzu HPLC System, Gerstel MPS 3C, And Peak Scientific Nitrogen Generator

This AB Sciex API 3200 LC/MS/MS liquid chromatography/mass spectrometry system includes an AB Sciex API 3200 series mass spectrometer, Shimadzu HPLC System, Gerstel MPS 3C, And Peak Scientific Gas Generator.

The API 3200 LC-MS/MS system is a fully-integrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, all in a compact package. It features the Turbo V ion source with plug-and-play probes that let you analize an extended range of compounds over a wide range of flow rates.

The innovative Turbo V ion source efficiently ionizes compounds over a wide range of flow rates, virtually eliminating cross-contamination, even with large sample loads. The patented LINAC collision cell technology delivers superior MS/MS performance allowing faster scan times without compromising sensitivity or mass spectral quality.

Ideal for a variety of applications including proteomics, drug discovery, drug development, contaminant testing, or forensic studies, the 3200 improves productivity by delivering a single-system solution with best-in-class technical performance.

 

Specifications:
Scanning Range	Quadrupole mode: 5 to 2000 m/z LIT mode: 50 to 1700 m/z
Dimensions	42″ W x 20″ D x 27″ H (106.7 x 50.8 x 68.6 cm)
Weight	330 lbs. (150 kg)
Detector Type	Pulse Counting CEM
Dynamic Range	5 orders of magnitude
Ionization Sources	TurboIonSpray, APCI, DuoSpray, PhotoSpray
Mass Accuracy / Stability	0.1 Da over 8 hours and 0.15 Da over 24 hours
Mass Range	5-1,700 (Quad); 50-1,700 (LIT)
Polarity Switching Time	700 ms
Scan Speed (Quad)	24,000 Da/sec
Scan Types	Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM, EMS, EPI, ER, MS3, TripleTrap
Operating Altitude	Up to 2000 m (6560 feet) above sea level
Ambient Temperature*	5°C (41°F) to 40°C (104°F)
Relative Humidity	80% for temperatures up to 31°C (88°F), decreasing lineraly to 50% at 40°C (104°F)
Mains Supply Voltage Fluctuations	±10% of the nominal voltage
Pollution Degree	Pollution Degree 2

*Over time, the temperature must remain within a range of 4 °C (7.2 °F), with the rate of the change in temperature not exceeding 2°C (3.6°F) per hour. Ambient temperature fluctuations exceeding the limits might result in mass shifts in spectra.

Features:

• The standard Turbo V ion source provides the plug-and-play TurboIonSpray probe and APCI probe, a durable ceramic interface, and compatibility with high flow rates
• Additional compatible ion sources, including the DuoSpray™ ion source, PhotoSpray ion source, and NanoSpray III ion source components, provide further flexibility
• Scanning in a mass range of m/z 5 to 2000 in quadrupole mode and m/z 50 to 1700 in LIT mode
• LINAC® collision cell eliminates cross-talk and allows for multi-component analysis during an LC run
• Integrated switching valve and integrated syringe pump provide convenient sample introduction flexibility
• Compatible with metabolite ID software, which automates metabolite identification, providing rapid, high-confidence results from experiments
• Safe fluids to be used include Methanol, acetonitrile, water, formic acid, and ammonium acetate

 

The Shimadzu Prominence HPLC System includes:

• CTO 20A Column Oven
• (2) LC-20AD Pumps
• DGU-20A3 Degasser
• CBM-20A Communications Bus Module
• FCV-11AL Valve Unit

 

The Shimadzu CTO-20A Column Oven is a forced air-circulation-type column oven. The CTO-20A allows you to regulate the temperature in a range going from 10 deg. C above room temperature to 85 deg. C. Using an electronic cooler, can regulate the temperature in a large range going from 10 deg. C below room temperature to 85 deg. C.

 

The Shimadzu LC-20AD Solvent Delivery Unit offers the most stable solvent delivery performance in the world. The unit achieves pulse-free solvent delivery with an automatic pulsation-correction mechanism and high-speed micro plunger drive. Flow-rate accuracy and precision in the micro-flow rate region have been improved with the addition of a new type of check valve and modifications to the solvent delivery control method. New and improved plunger seal materials ensure robust, long-lasting performance.

 

Solvent Delivery Unit Specifications:
Solvent Delivery Method	Parallel-type double plunger
Plunger Capacity	10µL
Maximum Discharge Pressure	40MPa
Flow-rate Setting Range	0.0001 to 10.0000 mL/min.
Flow-rate Accuracy	No more than 1% or 0.5µL/min., whichever is greater (0.01 to 2 mL/min.)
Flow-rate Precision	No more than 0.06% RSD or 0.02 min SD, whichever is greater
Pulsation	0.1 MPa (for water at 1.0 mL/min. and 7MPa)
Plunger Rinsing Mechanism	Manual rinsing or automatic rinsing using optional product
Safety Measures	Liquid-leakage sensor, high-pressure/low-pressure limits
Operating Temperature Range	4°C to 35°C
Dimensions	10.23″ W x 16.53″ D x 5.5″ H (26 x 42 x 14 cm)
Weight	22 lbs. (10 kg)
Power100 VAC, 150 VA, 50/60 Hz

 

Shimadzu DGU-20A3 Degasser Specifications:
Degassing Performance	UV absorption difference with and without degasser is over 300 mAU (using UV absorption detector, wavelength 210 nm, sensitivity 0.5 AU, flowing Methanol saturated with dissolved Oxygen (HPLC grade) at 25°C with 1 ml/min)
Number of flow lines	3
Max. Operating Flow Rate	20 mL/min per flow line (using deionised water – applicable range varies depending on the solvent being delivered)
Internal capacity	380µL per flow line
Pressure Resistance	98 kPa (1 kgf/cm2) or less (nly when suctioning with the delivery unit. Pressurized delivery to the degasser is prohibited.)
Tubing	3 mm O.D. x 1.5 mm I.D. PTFE tubing
Materials Contacting Solvents	Special synthetic polymers designed for all solvents (degassing membrane), PEEK (connector joints)
External Output	When system self-diagnostics detects an abnormality, a signal is output to the external terminal (ALARM)
Self-cleaning	Includes mechanism that extends vacuum pump life by drawing in air when the vacuum pump is running
Dimensions	10.24″ W x 16.54″ D x 2.83″ H (26 x 42 x 7.2 cm)
Weight	11 lbs. (5 kg)
Operating Conditions	4 – 35°C, 20 – 85% humidity
Power supply	+24 V (powered via connection by remote cable to delivery unit)

 

The Shimadzu CBM-20A Communications Bus Module is the world’s first Web-based HPLC system controller. With a data buffering function, the CBM-20A acts as an interface for connecting LC workstations, network-client computers, and analytical instruments via Ethernet.

Features:

• Set-up, control and monitor HPLC remotely
• Up to eight modules can be connected
• Equipped with Web-control functions it allows for system control, monitoring, and maintenance information via the internet
• Auto-purge, user intervention is not required to open or close the drain valve

 

Specifications:
Number of Connectable Units	8
Connectable Unit Capability	Solvent Delivery: 4 max Autosamplers: 1 Column Ovens: 1 Detectors: 2 (max.) Fraction Collectors: 1 Sub-controllers: 2 (max.)
Event I/O	4 inputs, 4 outputs
Analog Boards	Up to 2 boards can be mounted
Operating Temperature Range	4°C to 35°C
Dimensions	10.24″ W x 16.54″ D x 5.51″ H (26 x 42 x 14 cm)
Weight	12.13 lbs. (5.5 kg)
Power	100 VAC, 50/60HZ, 100VA

 

The Gerstel MPS 3C is a multifunctional autosampler and sample preparation robot for GC and GC/MS. The MPS provides modern analytical laboratories high throughput, high performance and unparalleled flexibility.

 

The Peak Scientific Nitrogen Generator, Model NM20ZA, is designed to operate LC/MS analyzers by producing the required rates, purities and pressures for source and exhaust gas. Utilizing separation technology to selectively remove oxygen, moisture and other gases.

Features:

• Reassurance – Generator performs 24/7, if required
• Durable – Few moving parts with minimum maintenance requirements
• Single Source Solution – One generator can cater for the requirements of multiple AB SCIEX LCMS
• Quiet – Completely silent in operation
• Mobile – Flexibility to position generator where required, wall- mountable for small labs
• Simple Installation – Generator only needs to be connected to the air source and application
• Economical – More cost effective than any other gas supply method
• Convenient – Gas on demand, no health hazards, no need to worry about running out of gas
• Technical Specification

 

Specifications:
Curtain Gas (Nitrogen)	5L/min @ 60psi
Source Gas (Zero Air)	22L/min @ 100psi
Exhaust Gas (Air)	8L/min @ 60psi
Outlet Pressure	System Set
Nitrogen Pressure Dewpoint	-40°C/-40°F
Particles	<0.01 µm
Suspended Liquids	None
Outlets	(3) 1/4″
Pressure Gauges	3
Phthalates	None
Internal Air Compressor	Yes
Noise Level	59db(A)
Power	230V, 50/60Hz 5.0A
Dimensions	37″ W x 27″ D x 24″ H (94 x 67 x 60 cm)
Weight	265 lbs. (120 kg)

 

System includes a Varian HS 602 dual-stage rotary vane pump, PC pre-loaded with software, monitor, keyboard, mouse and cables.

Please note: system includes vacuum pump.