The Amersham Biosciences LEADseeker Multimodality Imaging System is a completely automated system designed to include fluorescent assay screening. The LEADseeker Multimodality Imager includes an excitation light system consisting of a 150 W tungsten halogen lamp and ring light combined with automated excitation and emission filter changing facilities, enables up to six excitation wavelengths and four emission wavelengths to be selected. The other main component is a CCD camera composed of a grade 1, thinned and back-illuminated CCD chip mounted in a light-tight case and Borealis TM lens. The chip is cooled to -100 °C by using a proprietary refrigerant to minimize dark current (thermal) noise, and a vacuum prevents the formation of condensation. High resolution imaging is achieved by having an area of 24.6 mm x 24.6 mm (1024 x 1024 pixels). Within this set area it is possible to image, with appropriate instrument design, a surface area of 8 cm x 12 cm i.e. the footprint of a conventional microplate.
The LEADseeker Multimodality Imager can function in three modes: as a manual system for assay development, in a stacker workstation mode where microplates are delivered from a stacker carousel to the imager, or as a fully automated system integrated with major robotics platforms for high-throughput screening (HTS) environments. Plates can be imaged in seconds and automation allows the processing of several hundred plates, or approximately 500 000 tests per day, using miniaturized screening formats.
- Detection modes: radiometric, luminescence, fluorescence
- Wavelength range: 550 to 800 nm