The BD Biosciences Accuri C6 Flow Cytometer is easy to use and simple to maintain. Small and easily transportable, the Accuri C6 is small enough to easily fit on a benchtop, and can be placed in a laminar flow hood.
The system is equipped with a blue laser and a red laser, two scatter detectors, and four fluorescence detectors with interference filters optimized for the detection of FITC, PE, PerCP-Cy5.5, and APC. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. A unique low-pressure pumping system drives the fluidics. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration over 5 x 106 cells per mL.
BD Accuri C6 software has an intuitive user interface designed with the researcher in mind. The tabbed interface provides quick access to the collection, analysis, and statistics functions. Analysis can be performed on the BD Accuri C6 cytometer or can be exported into third-party programs such as FCS Express.
|Dimensions||14.75" W x 16.5" D x 11" H (37.5 x 41.9 x 27.9 cm)|
|Weight||30 lbs. (13.6 kg.)|
|Laser Excitation||488 nm (rated at 20,000-h life); 640 nm (rated at 20,000-h life)|
|Laser Profile||10 x 75 μm|
|Light Scatter Detection||Forward (0°, ±13°); Side (90°, ±13°)|
|Emission Detection||4 colors, user-changeable optical filters|
|Standard Filters Installed||FL1 533/30 nm (eg, FITC/GFP); FL2 585/40 nm (eg, PE/PI); FL3 >670 nm (eg, PerCP, PerCP-Cy5.5, PE-Cy7); FL4 675/25 nm (eg, APC)|
|Optical Alignment||Fixed alignment|
|Flow Cell||200 μm ID quartz capillary|
|Minimum Detectable Particle Size||0.5 μm|
|Minimum Sample Volume||50 μL|
|Pre-Set Flow Rates and Core Sizes||Slow : 14 μL/min, 10-μm core; Medium: 35 μL/min, 16-μm core; Fast: 66 μL/min, 22-μm core|
|Custom Sample Flow Rates||10–100 μL/min|
|Custom Core Diameter||5–40 μm|
|Recommended Sheath Fluid||0.2-μm filtered DI water|
|Maximum Events Per Sample||1 million events per well|
|Fluorescence Sensitivity, MESF*||FITC <150; PE <100|
|Scatter Resolution||Resolves human peripheral blood lymphocytes, monocytes, and granulocytes|
|Fluorescence Linearity||2 ±0.05% for chicken erythrocyte nuclei (CEN)|
|Fluorescence Precision||<3% CV for CEN|
|Data Acquisition Rate||10,000 events/second, maximum|
|Power Requirements||100–240 VAC, 50/60 Hz|
|Typical Power Consumption||154 VA|
|Heat Output||240 BTU/hour maximum output|
|Operating Ranges||15–30°C; <80% relative humidity|
|Signal Processing||24-bit datapath|
|Computer Interface||USB 2.0|
*MESF values determined using Thermo Scientific Cyto-Cal™ Multifluor Plus Violet Beads.