Category: Flow Cytometers | Subcategory: Cell Analysis

BD Biosciences Accuri C6 Flow Cytometer

BD Biosciences Accuri C6 Flow Cytometer Image

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BD Biosciences Accuri C6 Flow Cytometer ImageBD Biosciences Accuri C6 Flow Cytometer ImageBD Biosciences Accuri C6 Flow Cytometer ImageBD Biosciences Accuri C6 Flow Cytometer ImageBD Biosciences Accuri C6 Flow Cytometer ImageBD Biosciences Accuri C6 Flow Cytometer Image

The BD Biosciences Accuri C6 Flow Cytometer is easy to use and simple to maintain. Small and easily transportable, the Accuri C6 is small enough to easily fit on a benchtop, and can be placed in a laminar flow hood.

The system is equipped with a blue laser and a red laser, two scatter detectors, and four fluorescence detectors with interference filters optimized for the detection of FITC, PE, PerCP-Cy5.5, and APC. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. A unique low-pressure pumping system drives the fluidics. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration over 5 x 106 cells per mL.

BD Accuri C6 software has an intuitive user interface designed with the researcher in mind. The tabbed interface provides quick access to the collection, analysis, and statistics functions. Analysis can be performed on the BD Accuri C6 cytometer or can be exported into third-party programs such as FCS Express.

 

Specifications:
Dimensions14.75" W x 16.5" D x 11" H (37.5 x 41.9 x 27.9 cm)
Weight30 lbs. (13.6 kg.)
Laser Excitation488 nm (rated at 20,000-h life); 640 nm (rated at 20,000-h life)
Laser Profile10 x 75 μm
Light Scatter DetectionForward (0°, ±13°); Side (90°, ±13°)
Emission Detection4 colors, user-changeable optical filters
Standard Filters InstalledFL1 533/30 nm (eg, FITC/GFP); FL2 585/40 nm (eg, PE/PI); FL3 >670 nm (eg, PerCP, PerCP-Cy5.5, PE-Cy7); FL4 675/25 nm (eg, APC)
Optical AlignmentFixed alignment
Flow Cell200 μm ID quartz capillary
Minimum Detectable Particle Size0.5 μm
Minimum Sample Volume50 μL
Pre-Set Flow Rates and Core SizesSlow : 14 μL/min, 10-μm core; Medium: 35 μL/min, 16-μm core; Fast: 66 μL/min, 22-μm core
Custom Sample Flow Rates10–100 μL/min
Custom Core Diameter5–40 μm
Recommended Sheath Fluid0.2-μm filtered DI water
Maximum Events Per Sample1 million events per well
Fluorescence Sensitivity, MESF*FITC <150; PE <100
Scatter ResolutionResolves human peripheral blood lymphocytes, monocytes, and granulocytes
Fluorescence Linearity2 ±0.05% for chicken erythrocyte nuclei (CEN)
Fluorescence Precision<3% CV for CEN
Data Acquisition Rate10,000 events/second, maximum
Power Requirements100–240 VAC, 50/60 Hz
Typical Power Consumption154 VA
Heat Output240 BTU/hour maximum output
Operating Ranges15–30°C; <80% relative humidity
Signal Processing24-bit datapath
Computer InterfaceUSB 2.0

*MESF values determined using Thermo Scientific Cyto-Cal™ Multifluor Plus Violet Beads.

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