The BD Biosciences LSRFortessa X-20 Cell Analyzer With FACSFlow Supply System delivers high performance, multicolor analysis with the most compact footprint in its class at just 30 x 29 in. (76.2 X 73.7 cm) and a height of just 30 in. (76.2 cm).
The BD LSRFortessa X-20 features 5 lasers: blue, red, violet, yellow-green, and UV; enabling the detection of up to 20 parameters simultaneously. The unit fits easily on the benchtop for the cost-effective use of valuable laboratory space. In addition to the reduced size, design enhancements provide easier access to bandpass filters and mirrors, simplifying changes in the instrument configuration.
Excitation Optical Platform
The BD LSRFortessa X-20 optical layout allows for up to five lasers.
355 nm: 15 mW
405 nm: 50 mW
488 nm: 50 mW
561 nm: 50 mW
640 nm: 40 mW
Power loss at flow cell: <20% of specified laser power
Flow Cell Design
Rectangular quartz cuvette: Internal cross-section, 430 x 180 μm
External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light.
Fixed optical assembly with spatially separated laser beams.
The quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency.
Forward Scatter Detection
Photodiode detector with a 488/10 bandpass (BP) filter
Side Scatter Detector
Photomultiplier tube (PMT) with a 488/10 BP filter
Emission Optical Design
Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays.
The BD LSRFortessa X-20 uses configurable polygon-shaped optical pathways that use signal reflection to maximize signal detection.
FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
PE-Cy™5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5)
APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
FITC and PE measurements performed using SPHERO™ Rainbow Calibration Particles (RCP-30-5A)
PE-Cy5 and APC measurements performed using SPHERO™ Ultra Rainbow Calibration Particles (URCP38-2K)
Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
Forward and Side Scatter Sensitivity
Enables separation of fixed platelets from noise.
Forward and Side Scatter Resolution
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Side Scatter Resolution
Enables separation of 0.5-μm beads from noise.
Data Acquisition Rate
40,000 events/sec with beads
Sample Flow Rates: Front button panel provides three modes: RUN, STANDBY, and PRIME
Continuously adjustable flow rate, plus three preset flow rates: LO: 12 μL/min; MED: 35 μL/min; HI: 60 μL/min
Standard Fluidic Reservoirs: One 8-L sheath container and one 10-L waste container provided.
Data Management Software: BD FACSDiva™ v8.0 or later
Dimensions: 29" W x 30" D x 30" H inches (73.7 x 76.2 76.2 cm)
Weight: ~159 kg (~350 lb) depending on configuration
Temperature Operating Range: 19°C–26°C
Humidity: 10% to 90% relative, non-condensing
Heat Dissipation: ~2,700 BTU per hour depending on configuration
Air Supply: None required
Power: 100-230 V, 60 Hz. The unit (including computer system/printer) requires one dedicated circuit with a dedicated AC source not shared with any other equipment.
Maximum power: 1,500 watts
Noise: <70 dB
Compliance with Safety Standards: UL 61010 (US)