Category: HPLC - Liquid Chromatography | Subcategory: HPLC System

Eksigent NanoLC-Ultra 2D HPLC System

Eksigent NanoLC-Ultra 2D HPLC System Image

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Eksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System ImageEksigent NanoLC-Ultra 2D HPLC System Image

The Eksigent NanoLC-Ultra 2D HPLC System includes the Eksigent NanoLC-Ultra 2D, the NanoLC AS-2 Autosampler, ekspert cHiPLC, and iks-PS-250 DC Power Supply with DC24V/DC5V Output.

The higher pressure capability of the NanoLC-Ultra platform allows the use of longer columns and/or columns packed with smaller particles for higher resolution separations of complex (digested) protein samples. Moreover, the NanoLCUltra family features self-priming and self-purging pumps for easier changing of solvents, and a temperature-controlled column compartment for maintaining exceptional retention time reproducibility.

Eksigent’s proven Microfluidic Flow Control™ (MFC) technology guarantees reliable and reproducible results, while integration with major mass spectrometer software packages makes the system a seamless addition to almost any nanoLCMS workflow. A range of different NanoLC-Ultra system configurations are available for various proteomics applications—from single gradient systems for direct sample injection, to dual gradient systems for multidimensional separations, and dual column set-up for increased throughput.

The NanoLC-Ultra 1D system contains one binary gradient pump, allowing for direct on-column injections. The NanoLCUltra 1Dplus system adds an isocratic loading pump for sample enrichment experiments in which sample is loaded onto a trap column prior to analysis on a NanoLC column. NanoLC-Ultra 2D systems include all of the features of the 1D, plus the added capability of automated 2-dimensional chromatography. The NanoLC-Ultra 2D (two gradients) system is designed for advanced applications such as on-line 2D LC or high throughput setups using two nanoLC columns.

 

NanoLC-Ultra 2D Specifications:
Dimensions14" W x 24" D x 18" H (35.56 x 60.96 x 45.72 cm)
Weight (depending on configuration)Up to 70 lbs. (31.75 kg.)
Working Temperature15°C to 30°C
Power100-240 VAC, 400 VA
Maximum Pressure10,000 psi

Standard flow rate ranges

  • Loading pump
  • Nano gradient
  • Micro gradient

 

  • 1-30 µl/min
  • 50-500 nl/min (up to 1000 nl/min at reduced max press.)
  • 1-10 µl/min (up to 20 ul/min at reduced max press.)
Flow Rate Accuracy<1%
Flow Rate Precision<0.5% @ 200 nl/min
Gradient Accuracy<1% @ 200 nl/min
Gradient Delay<25 nl
Column ovenAmbient +5°C to 40°C (±1°C)
Built-in 10-port valve
  • 1/32" connection; 0.1 mm bore
  • Port-to-port volume <25 nl
  • Max. pressure 10,000 psi
  • Stainless Steel with proprietary coating on valve stator
Wetted PartsStainless Steel, Titanium, PEEK, Fused Silica, FEP, proprietary inert coating
I/O
  • 1 x USB for instrument control
  • 2 x RS232 serial output
  • Each pump channel
    • 2 x TTL in
    • 4 x TTL out
  • AD converter
Instrument ControlEksigent software with plug-ins for Thermo Electron's Xcalibur®, Bruker Daltonics' HyStar® and ABI/Sciex Analyst® software

 

The Eksigent AS-2 Autosampler can be operated by integrated Eksigent Control Software. The flexible and easy-to-use autosampler employs a 2.4 ul volume needle for injection of small volumes without sample losses. The needle is coated with a proprietary inert coating on both the inside and outside to prevent sample losses due to adsorption. The sample compartment is completely enclosed to ensure temperature control down to 4°C. Capacity is two microtiter plates or 96 vials. Using two different wash positions, the needle is washed inside and outside to reduce carry-over. The 6-port injection valve has port-to-port volumes of <25 nl, and is rated to 10,000 psi. A proprietary coating on the valve stator and rotor assures excellent valve reliability and reduces sample adsorption.

 

AS-2 AutosamplerSpecifications:
Dimensions14" W x 24" D x 14" H (35.56 x 60.96 x 35.56 cm)
Weight46 lbs. (20.87 kg.)
Working Temperature15° to 30°C
Power100-240 VAC; 200 VA
Maximum Pressure10,000 psi
CapacityTwo microtiter plates (96 high/low or 384 low) or 48/12 sample vial racks
Injection valve
  • 1/32 “ connection; 0.1 mm bore
  • Port-to-port volume < 25 nl
  • Max. pressure 10,000 psi
  • Stainless Steel with proprietary coating on valve stator and rotor
Injection VolumeProgrammable from 100 nl to 10 µl with standard 10 µl loop
Injection volume reproducibility
  • Full loop: RSD <0.8%
  • Partial loop: RSD <1%
  • µl-pick-up: RSD <2%
Carry-over<0.05%
Sample Tray Cooling4°C to ambient -3°C (±2 °C)
Wetted PartsStainless Steel, PTFE, PEEK, Glass, Teflon, TEFZEL, fused silica, proprietary inert coating
I/O
  • RS232 serial port for instrument control
  • 1 programmable relay output
  • 2 programmable TTL inputs
Instrument controlEksigent software with plug-ins for Thermo Electron's Xcalibur®, Bruker Daltonics' HyStar® and ABI/Sciex Analyst® software

 

The Eksigent ekspert cHiPLC nanoflex Docking Station is a chip-based platform that delivers sensitivity, column-to-column reproducibility and ease of operation when in combination with the NanoLC-Ultra 2 system.

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