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The Eksigent NanoLC-Ultra 2D HPLC System includes the Eksigent NanoLC-Ultra 2D, the NanoLC AS-2 Autosampler, ekspert cHiPLC, and iks-PS-250 DC Power Supply with DC24V/DC5V Output.

The higher pressure capability of the NanoLC-Ultra platform allows the use of longer columns and/or columns packed with smaller particles for higher resolution separations of complex (digested) protein samples. Moreover, the NanoLCUltra family features self-priming and self-purging pumps for easier changing of solvents, and a temperature-controlled column compartment for maintaining exceptional retention time reproducibility.

Eksigent’s proven Microfluidic Flow Control™ (MFC) technology guarantees reliable and reproducible results, while integration with major mass spectrometer software packages makes the system a seamless addition to almost any nanoLCMS workflow. A range of different NanoLC-Ultra system configurations are available for various proteomics applications—from single gradient systems for direct sample injection, to dual gradient systems for multidimensional separations, and dual column set-up for increased throughput.

The NanoLC-Ultra 1D system contains one binary gradient pump, allowing for direct on-column injections. The NanoLCUltra 1Dplus system adds an isocratic loading pump for sample enrichment experiments in which sample is loaded onto a trap column prior to analysis on a NanoLC column. NanoLC-Ultra 2D systems include all of the features of the 1D, plus the added capability of automated 2-dimensional chromatography. The NanoLC-Ultra 2D (two gradients) system is designed for advanced applications such as on-line 2D LC or high throughput setups using two nanoLC columns.

 

NanoLC-Ultra 2D Specifications:
Dimensions	14" W x 24" D x 18" H (35.56 x 60.96 x 45.72 cm)
Weight (depending on configuration)	Up to 70 lbs. (31.75 kg.)
Working Temperature	15°C to 30°C
Power	100-240 VAC, 400 VA
Maximum Pressure	10,000 psi
Standard flow rate ranges Loading pump Nano gradient Micro gradient	1-30 µl/min 50-500 nl/min (up to 1000 nl/min at reduced max press.) 1-10 µl/min (up to 20 ul/min at reduced max press.)
Flow Rate Accuracy	<1%
Flow Rate Precision	<0.5% @ 200 nl/min
Gradient Accuracy	<1% @ 200 nl/min
Gradient Delay	<25 nl
Column oven	Ambient +5°C to 40°C (±1°C)
Built-in 10-port valve	1/32" connection; 0.1 mm bore Port-to-port volume <25 nl Max. pressure 10,000 psi Stainless Steel with proprietary coating on valve stator
Wetted Parts	Stainless Steel, Titanium, PEEK, Fused Silica, FEP, proprietary inert coating
I/O	1 x USB for instrument control 2 x RS232 serial output Each pump channel 2 x TTL in 4 x TTL out AD converter
Instrument Control	Eksigent software with plug-ins for Thermo Electron's Xcalibur®, Bruker Daltonics' HyStar® and ABI/Sciex Analyst® software

 

The Eksigent AS-2 Autosampler can be operated by integrated Eksigent Control Software. The flexible and easy-to-use autosampler employs a 2.4 ul volume needle for injection of small volumes without sample losses. The needle is coated with a proprietary inert coating on both the inside and outside to prevent sample losses due to adsorption. The sample compartment is completely enclosed to ensure temperature control down to 4°C. Capacity is two microtiter plates or 96 vials. Using two different wash positions, the needle is washed inside and outside to reduce carry-over. The 6-port injection valve has port-to-port volumes of <25 nl, and is rated to 10,000 psi. A proprietary coating on the valve stator and rotor assures excellent valve reliability and reduces sample adsorption.

 

AS-2 AutosamplerSpecifications:
Dimensions	14" W x 24" D x 14" H (35.56 x 60.96 x 35.56 cm)
Weight	46 lbs. (20.87 kg.)
Working Temperature	15° to 30°C
Power	100-240 VAC; 200 VA
Maximum Pressure	10,000 psi
Capacity	Two microtiter plates (96 high/low or 384 low) or 48/12 sample vial racks
Injection valve	1/32 “ connection; 0.1 mm bore Port-to-port volume < 25 nl Max. pressure 10,000 psi Stainless Steel with proprietary coating on valve stator and rotor
Injection Volume	Programmable from 100 nl to 10 µl with standard 10 µl loop
Injection volume reproducibility	Full loop: RSD <0.8% Partial loop: RSD <1% µl-pick-up: RSD <2%
Carry-over	<0.05%
Sample Tray Cooling	4°C to ambient -3°C (±2 °C)
Wetted Parts	Stainless Steel, PTFE, PEEK, Glass, Teflon, TEFZEL, fused silica, proprietary inert coating
I/O	RS232 serial port for instrument control 1 programmable relay output 2 programmable TTL inputs
Instrument control	Eksigent software with plug-ins for Thermo Electron's Xcalibur®, Bruker Daltonics' HyStar® and ABI/Sciex Analyst® software

 

The Eksigent ekspert cHiPLC nanoflex Docking Station is a chip-based platform that delivers sensitivity, column-to-column reproducibility and ease of operation when in combination with the NanoLC-Ultra 2 system.