ForteBio Octet RED96e System

The ForteBio Octet RED96e System is an advanced, label-free, real-time biosensor platform designed for high-throughput analysis of biomolecular interactions. Utilizing Bio-Layer Interferometry (BLI) technology, it enables precise quantification of binding kinetics, affinity, and concentration without the need for microfluidics, making it ideal for applications in biopharmaceutical development, quality control, and academic research.

Key Features & Benefits:

• Real-Time Label-Free Detection: Eliminates the need for fluorescent or radioactive labels, streamlining assay development and preserving sample integrity.
• High Throughput Analysis: Simultaneously analyzes up to 8 samples with automated 96-well plate compatibility, enabling rapid data collection for large-scale studies.
• Flexible Sample Handling: Open microplate format supports direct dipping of biosensors, reducing consumable use and increasing workflow efficiency.
• Precise Kinetic Measurements: Accurately quantifies association and dissociation rates with broad dynamic range and low baseline drift.
• Intuitive Software: Octet Software offers streamlined data acquisition and analysis, with options for quantitation, kinetic fitting, and epitope binning.

Specifications:

• Detection Technology: Bio-Layer Interferometry (BLI)
• Sample Format: 96-well microplate
• Channels: 8 independent channels
• Sample Volume: 180–220 µL per well
• Analysis Temperature Range: 15–40 °C, adjustable in 1 °C increments
• Orbital Shaking Speed: Static or 100–1500 rpm
• Association Rate Constant (ka): 10¹ to 10⁷ M⁻¹s⁻¹
• Dissociation Rate Constant (kd): 10⁻⁶ to 0.1 s⁻¹
• Affinity Constant (KD): 1 mM to 10 pM
• Baseline Noise: ≤ 3.5 pm (RMS)
• Baseline Drift: ≤ 0.1 nm/hour
• Quantitation Range (Human IgG with Protein A Biosensor): 0.05–2000 µg/mL
• Analysis Time: 2 minutes for 8 samples; ≤ 32 minutes for 96 samples
• Dimensions (H × W × D): 49 cm × 56 cm × 46 cm (19.5 in × 22 in × 18.2 in)
• Weight: 32.7 kg (72 lb)
• Electrical Requirements: 100–120/200–240 VAC, 50/60 Hz, 4 A max
• Power Consumption: 200 W (300 W peak)
• Operating Systems: Windows® 10 Professional (64-bit), Windows 7 Professional (32/64-bit)
• Compliance: CE, Nemko

With its high sensitivity and flexibility, the Octet RED96e System streamlines workflows for kinetic analysis, quantitation, and epitope binning, supporting a wide range of applications from small molecule screening to cell line development.