Miltenyi Biotec MACSQuant 16 Flow Cytometer

The Miltenyi Biotec MACSQuant 16 Flow Cytometer offers high-resolution multiparametric analysis, enabling simultaneous detection and analysis of up to 16 parameters from a single sample. Its state-of-the-art optical design and advanced electronics ensure outstanding sensitivity and resolution, empowering researchers to unlock deeper insights from their samples.

With an intuitive user interface and automated features, the Miltenyi Biotec MACSQuant 16 Flow Cytometer streamlines workflows and simplifies operation, saving valuable time and resources. The instrument supports a wide range of sample types, from cells and microorganisms to subcellular particles, enabling versatile applications across diverse fields such as immunology, cancer research, stem cell biology, and drug discovery.

One of the key highlights of the Miltenyi Biotec MACSQuant 16 Flow Cytometer is its integrated magnetic cell separation technology, allowing seamless isolation of target cells prior to analysis. This unique feature enhances sample purity and minimizes background noise, ensuring accurate and reliable results

Features:

• Full automation – Load your samples, import your experiment settings, and leave the staining, data acquisition and analysis to the MACSQuant Analyzer 16
• Expanded fluorescent channels – Amplify your data with a selection from 14 fluorescence channels and an average of 40% less sample volume required.
• Flexible assay setup – Easily and immediately switch between tubes and plates at the click of a button.
• Multi-instrument alignment – Harmonize data with collaborating labs to ensure reproducibility.

Specifications:

Optics, Laser excitation, spatially separated:
405 nm, 65 mW, 488 nm, 50 mW, 640 nm, 72 mW
Emission detectors:
FSC: 488/10 nm, SSC: 405/10 nm, B1: 525/50 nm, B2: 579/34 nm, B3: 615/20 nm, B4: 667/30 nm
B5: 725/40 nm, B6: 785/62 nm, V1: 450/50 nm, V2: 525/50 nm, V3: 579/34 nm, V4: 615/20 nm
V5: 667/30 nm, R1: 667/30 nm, R2: 725/40 nm R3: 785/62 nm
Fluorescence sensitivity and resolution: MESFs (CV <5%), FITC < 110, PE < 75, APC < 100
Flow cell dimensions: 200×250 μm
Fluorescence detectors: Optimized with spectrally matched PMTs for all channels
Minimal uptake volume: 1 μL (25 μL recommended for volumetric counting applications)
Sample flow rate: 25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second
Measurement speed: <25 minutes per 96-well plate (5 μL measurement volume per well)
Sample uptake:1–450 µL
Maximal event rate: Up to 15,000 events/second
Footprint: 385.5 × 284.5 mm (15.18 × 11.2 in)
Height (adjustable touchscreen): 26.34 × 15.75 in (66.9 × 400 mm (26.34 × 15.75 in)
Size with miniSampler Plus (width × depth): 26.34 × 19.69 in (66.9×50 cm)
Weight:50 kg (110 lbs)
Monitor:15.6″ LCD touchscreen
Power requirements:100–240 V~, 50/60 Hz
Power consumption (standard use):450 W
RAM:8 GB DDR4 (SO-DIMM)
Mass storage:500 GB SSD
Ports:4× USB 2.0 ports, 6× USB 3.0 ports (2 at display),
2× DisplayPort, 2× LAN, DVI, RE-232, Audio
Emission sound pressure level at workstation:<61 dB(A)
Sample carry-over:0.01%
Fluorescence performance: 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales
Operating system: Embedded operating system
Measurement parameters: Area, width, height for all parameters, with time and volume
Signal processing: >18 bit dynamic range in area with 32 bit floating point signal processing
Compensation: Automated, or manual with 14×14 matrix, during or after acquisition
Data files: .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible)