The Molecular Devices Gemini XS Fluorescent Microplate Reader allows for fast, high-sensitivity measurements. It offers a flexible filter-free environment allowing the determination of optimal excitation and emission settings in 1.0 nm increments providing the best level of sensitivity for variable assay conditions. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Not only is this capable of high reproducibility, but offers RFU comparison between samples, temperature monitoring, correct reader settings, and sample mixing through auto mix.
- Dual Monochromators
- The right pair of excitation and emission wavelengths is always available because the dual monochromators allow the selection of any wavelength in 1 nm increments. New fluorophores can easily be evaluated without purchasing additional filters.
- Wavelength Scanning
- The most sensitive results are achieved by using optimal excitation and emission wavelengths. Literature wavelengths are often based on results from wavelength-limited, filter-based readers. Wavelength scanning ensures that the most sensitive assay conditions are used.
- Well Scanning
- Gemini XS can report a single point from the well center, or multiple data points from large well tissue culture plates. This provides high sensitivity for cell-based assays.
- Auto PMT Gain
- Because a single microplate often presents a range of fluorescence intensities greater than three orders of magnitude, Gemini XS features "Auto PMT Gain" to avoid saturating the photomultiplier tube. The signal is calibrated against an internal standard, so the reported RFU values of individual samples can be accurately compared.
|Microplate Formats||6-, 12-, 24-, 48-, 96- and 384|
|Light Source||Xenon flash lamp|
|Read Time||96 < 27 sec, 384 < 45 sec|
|Shaker Time||0 to 999 seconds|
|Fluorescence Photometric Performance:|| |
|Dual Monochromators||1nm increments|
|Bandwidth (Excitation & Emission)||9 nm|
|Time Resolved Fluorescence:|| |
|Data Collection||50-1450 µ/sec, 200µ/sec increments|
|Sensitivity||0.5 per well|
|Luminescence Performance:|| |
|Detection Limit||10 amol/well Alkaline Phosphate 200 µl/well|