Category: Microplate Readers | Subcategory: Multimode Readers

Molecular Devices SpectraMax M2 Microplate Reader

Molecular Devices SpectraMax M2 Microplate Reader Image

ITEM: 22766 In Stock

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Molecular Devices SpectraMax M2 Microplate Reader ImageMolecular Devices SpectraMax M2 Microplate Reader ImageMolecular Devices SpectraMax M2 Microplate Reader ImageMolecular Devices SpectraMax M2 Microplate Reader ImageMolecular Devices SpectraMax M2 Microplate Reader Image

The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor allow homogeneous and eterogeneous microplate assays to be performed in one flexible system. SpectraMax M2 readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.

The SpectraMax M2 reader uses two scanning monochromators to determine optimal excitation and emission settings. Changing methods or fluorophores requires only a few mouse selections to optimize the system.

Features:

  • Multi-detection capability
  • Top- and bottom-read (M2e only)
  • Dual-mode cuvette port
  • Dual monochromators
  • PathCheck and well volume sensor
  • Instrument and software validation
  • Robot compatible
Absorbance Photometric Performance Specifications:
Wavelength SelectionMonochromator, tunable in 1.0 nm increments
Wavelength Range200-1000 nm
Wavelength Bandwidth<4.0 nm
Wavelength Accuracy±2.0 nm
Wavelength Repeatability±0.2 nm
Photometric Range0-4.0 OD
Photometric Resolution0.001 OD
Photometric Accuracy (Microplate)<±0.006 OD, ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette)<±0.005 OD, ±1.0%, 0-2 OD
Photometric Precision<±0.003 OD, ±1.0%, 0-2 OD
Baseline Flatness<0.001 OD
Stray Light<0.05% @ 230 nm

 

Fluorescence Photometric Performance Specifications:
Dual Monochromators1 nm increment selection
EX Wavelength Range250–850 nm
EM Wavelength Range360–850 nm
Wavelength Bandwidth(EX, EM) 9 nm
Top-read Optimized Sensitivity5 pM (fluorescein) in 96 wells
Top-read Guaranteed* Sensitivity15 pM (fluorescein) in 96 wells


Time-Resolved Fluorescence (Secondary Mode) Specifications:
EX Wavelength Range250–850 nm
EM Wavelength Range360–850 nm
Data Collection50–1450 µsec., 200 µsec. increments
Guaranteed Sensitivity*7 pM Eu-chelate

 

Luminescence (Secondary Mode) Specifications:
Wavelength Range (M2)360–850 nm
Wavelength Range (M2e)250–850 nm
Guaranteed Sensitivity*50 fM alkaline phosphatase

 

General Photometric Performance Specifications:
Plate Formats6, 12, 24, 48, 96, 384 wells
Light SourceXenon flash lamp (1 joule/flash)
DetectorPhotomultiplier (R-3896)
Read Time96-well: Abs 18 sec., FI 15 sec.
384-well: Abs 49 sec., FI 45 sec.
Shaker Time0 to 999 seconds
Temperature RangeAmbient +4°C to 45°C
Temperature Uniformity±0.5°C @ 37°C set pt.
Temperature Accuracy±1°C @ 37°C

 

General Specifications:
Dimensions22.8" W x 15" D x 8.6" H (58 x 38 x 22 cm)
Weight35 lbs. (15.9 kg)
Power100-240V, 50/60Hz, 3A, 125W
Robot CompatibleYes

 

Applications:

  • DNA/RNA/protein quantitation and purity
  • PicoGreen/NanoOrange/Bradford
  • ELISAs/enzyme kinetics (i.e., Km, Ki, etc.)
  • Drug dissolution profiles
  • Live/Dead Viability/cytotoxicity assays
  • Caspase-3 and protease assays
  • cAMP assays using CatchPoint Assay Kits
  • Low volume applications

* For properly functioning, operated, and maintained equipment.
** Measurement type may extend read time

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