ALT ITEM: 24108 | SpectraMax M2e Multimode Microplate Reader

Category: Microplate Readers | Subcategory: Multimode Readers

Molecular Devices SpectraMax M2e Multimode Microplate Reader

ITEM: 24108 In Stock

The Molecular Devices SpectraMax M2e Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor allow homogeneous and eterogeneous microplate assays to be performed in one flexible system. SpectraMax M2e readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.

The SpectraMax M2 reader uses two scanning monochromators to determine optimal excitation and emission settings. Changing methods or fluorophores requires only a few mouse selections to optimize the system.

Features:

Multi-detection capability
Top- and bottom-read (M2e only)
Dual-mode cuvette port
Dual monochromators
PathCheck and well volume sensor
Instrument and software validation
Robot compatible

Absorbance Photometric Performance Specifications:
Wavelength Selection	Monochromator, tunable in 1.0 nm increments
Wavelength Range	200-1000 nm
Wavelength Bandwidth	<4.0 nm
Wavelength Accuracy	±2.0 nm
Wavelength Repeatability	±0.2 nm
Photometric Range	0-4.0 OD
Photometric Resolution	0.001 OD
Photometric Accuracy (Microplate)	<±0.006 OD, ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette)	<±0.005 OD, ±1.0%, 0-2 OD
Photometric Precision	<±0.003 OD, ±1.0%, 0-2 OD
Baseline Flatness	<0.001 OD
Stray Light	<0.05% @ 230 nm

Fluorescence Photometric Performance Specifications:
Dual Monochromators	1 nm increment selection
EX Wavelength Range	250–850 nm
EM Wavelength Range	250–850 nm
Wavelength Bandwidth	(EX, EM) 9 nm
Top-read Optimized Sensitivity	5 pM (fluorescein) in 96 wells
Top-read Guaranteed* Sensitivity	15 pM (fluorescein) in 96 wells
Bottom-read Optimized Sensitivity (M2e only)	10 pM (fluorescein) in 96 wells
Bottom-read Guaranteed* Sensitivity (M2e only)	25 pM (fluorescein) in 96 wells

Time-Resolved Fluorescence (Secondary Mode) Specifications:
EX Wavelength Range	250–850 nm
EM Wavelength Range	M2e: 250–850 nm
Data Collection	50–1450 µsec., 200 µsec. increments
Guaranteed Sensitivity*	7 pM Eu-chelate

Luminescence (Secondary Mode) Specifications:
Wavelength Range (M2)	360–850 nm
Wavelength Range (M2e)	250–850 nm
Guaranteed Sensitivity*	50 fM alkaline phosphatase

General Photometric Performance Specifications:
Plate Formats	6, 12, 24, 48, 96, 384 wells
Light Source	Xenon flash lamp (1 joule/flash)
Detector	Photomultiplier (R-3896)
Read Time	96-well: Abs 18 sec., FI 15 sec. 384-well: Abs 49 sec., FI 45 sec.
Shaker Time	0 to 999 seconds
Temperature Range	Ambient +4°C to 45°C
Temperature Uniformity	±0.5°C @ 37°C set pt.
Temperature Accuracy	±1°C @ 37°C

General Specifications:
Dimensions	22.8" W x 15" D x 8.6" H (58 x 38 x 22 cm)
Weight	35 lbs. (15.9 kg)
Power	100-240V, 50/60Hz, 3A, 125W
Robot Compatible	Yes

Applications: