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Category: Microplate Readers | Subcategory: Multimode Readers | Manufacturer: Molecular Devices
Molecular Devices SpectraMax M2e Multimode Microplate Reader
ITEM: 24108 In Stock
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The Molecular Devices SpectraMax M2e Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor allow homogeneous and eterogeneous microplate assays to be performed in one flexible system. SpectraMax M2e readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.
The SpectraMax M2 reader uses two scanning monochromators to determine optimal excitation and emission settings. Changing methods or fluorophores requires only a few mouse selections to optimize the system.
Features:
- Multi-detection capability
- Top- and bottom-read (M2e only)
- Dual-mode cuvette port
- Dual monochromators
- PathCheck and well volume sensor
- Instrument and software validation
- Robot compatible
| Absorbance Photometric Performance Specifications: | |
| Wavelength Selection | Monochromator, tunable in 1.0 nm increments |
| Wavelength Range | 200-1000 nm |
| Wavelength Bandwidth | <4.0 nm |
| Wavelength Accuracy | ±2.0 nm |
| Wavelength Repeatability | ±0.2 nm |
| Photometric Range | 0-4.0 OD |
| Photometric Resolution | 0.001 OD |
| Photometric Accuracy (Microplate) | <±0.006 OD, ±1.0%, 0-2 OD |
| Photometric Accuracy (Cuvette) | <±0.005 OD, ±1.0%, 0-2 OD |
| Photometric Precision | <±0.003 OD, ±1.0%, 0-2 OD |
| Baseline Flatness | <0.001 OD |
| Stray Light | <0.05% @ 230 nm |
| Fluorescence Photometric Performance Specifications: | |
| Dual Monochromators | 1 nm increment selection |
| EX Wavelength Range | 250–850 nm |
| EM Wavelength Range | 250–850 nm |
| Wavelength Bandwidth | (EX, EM) 9 nm |
| Top-read Optimized Sensitivity | 5 pM (fluorescein) in 96 wells |
| Top-read Guaranteed* Sensitivity | 15 pM (fluorescein) in 96 wells |
| Bottom-read Optimized Sensitivity (M2e only) | 10 pM (fluorescein) in 96 wells |
| Bottom-read Guaranteed* Sensitivity (M2e only) | 25 pM (fluorescein) in 96 wells |
| Time-Resolved Fluorescence (Secondary Mode) Specifications: | |
| EX Wavelength Range | 250–850 nm |
| EM Wavelength Range | M2e: 250–850 nm |
| Data Collection | 50–1450 µsec., 200 µsec. increments |
| Guaranteed Sensitivity* | 7 pM Eu-chelate |
| Luminescence (Secondary Mode) Specifications: | |
| Wavelength Range (M2) | 360–850 nm |
| Wavelength Range (M2e) | 250–850 nm |
| Guaranteed Sensitivity* | 50 fM alkaline phosphatase |
| General Photometric Performance Specifications: | |
| Plate Formats | 6, 12, 24, 48, 96, 384 wells |
| Light Source | Xenon flash lamp (1 joule/flash) |
| Detector | Photomultiplier (R-3896) |
| Read Time | 96-well: Abs 18 sec., FI 15 sec. 384-well: Abs 49 sec., FI 45 sec. |
| Shaker Time | 0 to 999 seconds |
| Temperature Range | Ambient +4°C to 45°C |
| Temperature Uniformity | ±0.5°C @ 37°C set pt. |
| Temperature Accuracy | ±1°C @ 37°C |
| General Specifications: | |
| Dimensions | 22.8″ W x 15″ D x 8.6″ H (58 x 38 x 22 cm) |
| Weight | 35 lbs. (15.9 kg) |
| Power | 100-240V, 50/60Hz, 3A, 125W |
| Robot Compatible | Yes |
Applications:
- DNA/RNA/protein quantitation and purity
- PicoGreen/NanoOrange/Bradford
- ELISAs/enzyme kinetics (i.e., Km, Ki, etc.)
- Drug dissolution profiles
- Live/Dead Viability/cytotoxicity assays
- Caspase-3 and protease assays
- cAMP assays using CatchPoint Assay Kits
- Low volume applications
* For properly functioning, operated, and maintained equipment.
** Measurement type may extend read time
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