Category: Electrophoresis | Subcategory: Electrophoresis System

ProteinSimple Milo Single-Cell Western Blot Platform

ProteinSimple Milo Single-Cell Western Blot Platform Image

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ProteinSimple Milo Single-Cell Western Blot Platform ImageProteinSimple Milo Single-Cell Western Blot Platform ImageProteinSimple Milo Single-Cell Western Blot Platform ImageProteinSimple Milo Single-Cell Western Blot Platform ImageProteinSimple Milo Single-Cell Western Blot Platform Image

The ProteinSimple Milo is the world's first Single-Cell Western platform. He measures protein expression in thousands of single cells in a single run so you can profile heterogeneity in your samples. Just load your cell suspension and the scWest chip captures ~1,000 single-cells. Milo then does a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Then just probe with your favorite conventional Western antibodies to measure `12 proteins per cell using a variety of multiplexing strategies. Use Single-Cell Westerns to unlock the single-cell proteome and measure more of the proteome than is possible with any other single-cell technique.

 

Specifications:
Sample TypeSuspension containing >10,000 cells
Cell Diameter7-25 µm in suspension
Cell TypeMammalian cells; globular in suspension and unfixed
Antibody RequirementStandard unlabeled primaries and fluorescent secondaries
Other Equipment Needed*Open-format fluorescence microarray scanner capable of 5 µm resolution
Molecular Weight (MW) Range15-175 kDa
MW Resolution10% differences in distinct spectral channels, as low as 30% differences in same spectral channel
Typical Target MultiplexingUp to four proteins per cell by spectral and size-based multiplexing Twelve - plus proteins per cell using stripping & reprobing
Workflow time4-6 hours
Dimensions**11" W x 13.25" D x 6.5" H (28 x 34 x 31 cm)
Height (Lid Open)12" (31 cm)
Weight (approx.)12 lbs. (5.5 kg)
Power100-240VAC, 50/60Hz, 2A/1A max. rated input current
HV Circuit Rating300V, 2.5A max

*Not included.
**Allow ~18" (46 cm) clearance for cables and ventilation.

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