Category: Electrophoresis | Subcategory: Electrophoresis System
ProteinSimple  WES Western Blot System
ProteinSimple  WES Western Blot System
ProteinSimple  WES Western Blot System
ProteinSimple  WES Western Blot System
ProteinSimple  WES Western Blot System
ProteinSimple  WES Western Blot System
ProteinSimple  WES Western Blot System
ProteinSimple  WES Western Blot System

ProteinSimple WES Western Blot System

ITEM: 32506 In Stock
Price: $15,950.00
Finance for as low as: $355.69/mo
Free 90 Day Warranty
One Year Upgrade Available
Tested & Serviced Prior to Shipping

The ProteinSimple WES Western Blot System is designed to perform Western blotting assays in a streamlined and efficient manner. Western blotting is a widely used laboratory technique for detecting and quantifying specific proteins in a complex mixture, such as cell lysates or tissue extracts.

Key Features:

  • Automation: The WES system automates many of the labor-intensive steps involved in traditional Western blotting, including gel electrophoresis, protein transfer, and antibody incubation. This automation reduces hands-on time and minimizes the risk of user-induced errors, ensuring more consistent and reliable results.
  • Microfluidic Capillary Electrophoresis: Instead of traditional gel electrophoresis, the WES system utilizes microfludic capillary electrophoresis technology to separate proteins by size. This approach provides excellent resolution and requires significantly less sample and reagents.
  • Rapid Results: The WES system can generate Western blot data in just a few hours, compared to the traditional method, which may take a full day or more to complete.
  • Quantitative Analysis: The platform offers quantitative data analysis, allowing researchers to accurately measure protein expression levels. It provides digital, objective results that eliminate the need for manual gel interpretation.
  • Minimal Sample Requirements: The WES system requires only a small amount of sample (typically 3-5 µg of total protein) and is compatible with a variety of sample types, including cell lysates, tissue homogenates, and more.
  • Simple Workflow: The workflow consists of loading samples, running the assay, and analyzing the results using the accompanying software. The intuitive software provides automatic quantification and data analysis, making it accessible to both experienced researchers and those new to Western blotting.
  • Multiplexing: The system allows for multiplexing, enabling the detection of multiple proteins in a single experiment. This is particularly useful for studying protein interactions and signaling pathways.
  • Reproducibility: The automated nature of the WES system ensures high reproducibility between experiments and different users.

The ProteinSimple WES Western Blot System is commonly used for biomarker discovery, protein expression profiling, and pathway analysis. Its automation, speed, and quantitative capabilities have made Western blotting more efficient and accessible, advancing research in areas such as cancer biology, immunology, and neuroscience.

Specifications:

Dimensions: 13″ W x 13″ D x 20.4 ” H inches (33 x 33 x 52 cm)
Max. samples per run: 25
Run time: < 3 hours
Throughput per day: Up to 75 samples
Power: 115 V, 60 Hz

DescriptionTotal Protein SpecificationImmunoassay Charge Specification
Sample Required0.3-1.2 µg lysate.6-1.2 µg
Volume Required3 µL/well3 µL/well
Size or pI RangeMolecular weight (MW) ladders range from 2-440 kDaMolecular weight (MW) ladders range from 2-440 kDa
Sizing CV<10%<10%
Intra-assay CV<15%<15%
Inter-assay CV<20%<20%
Resolution (± percent difference in MW)± 15-20% for MW <20 kDa
±10 % for MW > 20 kDa
± 15-20% for MW <20 kDa
±10 % for MW > 20 kDa
Quantitation CV<20%<20%
Dynamic Range2-3 logs3 logs
SensitivityngLow pg
Capillary5 cm, 100 µm, 400 nL 

 

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