Tangential Flow Filtration (TFF)

Tangential flow filtration (TFF), also known as cross-flow filtration, is a powerful separation technique used in various laboratory and bioprocessing applications. Unlike traditional "dead-end" filtration, TFF operates by circulating the sample fluid tangentially across a semi-permeable membrane. This continuous flow prevents membrane fouling and allows for efficient separation of target molecules based on size, molecular weight, or other characteristics. TFF systems are valuable for concentrating, diafiltering, and fractionating biological samples like proteins, antibodies, viruses, and cell cultures. This filtration method supports workflows in protein purification, cell harvesting, vaccine production, and wastewater treatment. Researchers and scientists often utilize TFF in conjunction with downstream analytical techniques such as spectroscopy, chromatography, and electrophoresis. Leading manufacturers of TFF systems... + More

Tangential flow filtration (TFF), also known as cross-flow filtration, is a powerful separation technique used in various laboratory and bioprocessing applications. Unlike traditional "dead-end" filtration, TFF operates by circulating the sample fluid tangentially across a semi-permeable membrane. This continuous flow prevents membrane fouling and allows for efficient separation of target molecules based on size, molecular weight, or other characteristics. TFF systems are valuable for concentrating, diafiltering, and fractionating biological samples like proteins, antibodies, viruses, and cell cultures. This filtration method supports workflows in protein purification, cell harvesting, vaccine production, and wastewater treatment. Researchers and scientists often utilize TFF in conjunction with downstream analytical techniques such as spectroscopy, chromatography, and electrophoresis. Leading manufacturers of TFF systems include MilliporeSigma, Sartorius, Pall Corporation, and Thermo Fisher Scientific, offering various models like Pellicon, Vivaflow, and Spectrum KrosFlo systems designed to cater to diverse research and industrial needs. These systems are commonly employed in sample preparation for research areas like proteomics, genomics, and drug discovery, providing a reliable and efficient means of separating and purifying valuable biological materials. - Less