GPC/SEC System

Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) systems are essential tools for analyzing the molecular weight distribution and size of polymers, proteins, and other macromolecules. These systems separate molecules based on their hydrodynamic volume as they pass through a column packed with porous beads. A GPC/SEC system typically consists of a pump, injector, column(s), detector(s), and data analysis software. By measuring the elution time and comparing it to known standards, researchers can determine the molecular weight and size distribution of their samples. This information is crucial for understanding material properties, optimizing synthesis processes, and characterizing complex mixtures. GPC/SEC workflows often integrate with other analytical techniques, such as light scattering, viscometry, and mass spectrometry,... + More

Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) systems are essential tools for analyzing the molecular weight distribution and size of polymers, proteins, and other macromolecules. These systems separate molecules based on their hydrodynamic volume as they pass through a column packed with porous beads. A GPC/SEC system typically consists of a pump, injector, column(s), detector(s), and data analysis software. By measuring the elution time and comparing it to known standards, researchers can determine the molecular weight and size distribution of their samples. This information is crucial for understanding material properties, optimizing synthesis processes, and characterizing complex mixtures. GPC/SEC workflows often integrate with other analytical techniques, such as light scattering, viscometry, and mass spectrometry, providing comprehensive macromolecular characterization. Leading manufacturers like Agilent, Waters, and Malvern Panalytical offer a range of GPC/SEC systems and accessories to meet diverse application needs. GPC/SEC systems are widely used in various scientific disciplines, including polymer chemistry, biochemistry, and pharmaceuticals. They are instrumental in polymer characterization, determining molecular weight averages, polydispersity index, and branching. In biopharmaceutical research, GPC/SEC is used to analyze protein aggregation, determine the purity of biotherapeutics, and study protein-protein interactions. Furthermore, GPC/SEC supports sample preparation for subsequent analyses like mass spectrometry and helps researchers understand the impact of molecular weight and size on material properties. Systems from manufacturers like Thermo Fisher and Shimadzu offer advanced features like automated sample handling, temperature control, and multiple detectors, enhancing the accuracy and efficiency of GPC/SEC analysis. ALT offers a selection of pre-owned and refurbished GPC/SEC systems, each backed by a standard 90-day warranty that can be upgraded to a three-year warranty, along with ALT's lifetime support. Because they're refurbished, GPC/SEC systems from ALT are typically priced 50%-80% lower than the cost of new instruments, helping labs stretch their budget further without compromising on quality. - Less