Flow Cytometers

Flow cytometers are sophisticated instruments essential for cell analysis and sorting in various scientific disciplines. They provide quantitative and qualitative data about individual cells and particles within a heterogeneous population. These instruments operate on the principles of fluid dynamics, optics, and electronics. A sample containing cells or particles is injected into a stream of sheath fluid, which focuses the cells into a single file as they pass through a laser beam. As each cell interacts with the laser, light is scattered and emitted fluorescence is detected by photomultiplier tubes (PMTs) or other detectors. This data is then processed to generate information about the cell's size, granularity (internal complexity), and the presence of specific markers labeled... + More

Flow cytometers are sophisticated instruments essential for cell analysis and sorting in various scientific disciplines. They provide quantitative and qualitative data about individual cells and particles within a heterogeneous population. These instruments operate on the principles of fluid dynamics, optics, and electronics. A sample containing cells or particles is injected into a stream of sheath fluid, which focuses the cells into a single file as they pass through a laser beam. As each cell interacts with the laser, light is scattered and emitted fluorescence is detected by photomultiplier tubes (PMTs) or other detectors. This data is then processed to generate information about the cell's size, granularity (internal complexity), and the presence of specific markers labeled with fluorescent dyes. Flow cytometry workflows involve sample preparation, staining with fluorescently labeled antibodies or dyes, data acquisition using the flow cytometer, and data analysis using specialized software. These workflows support a range of techniques, including immunophenotyping, cell cycle analysis, apoptosis assays, and cell sorting. Common applications span immunology, cell biology, oncology, microbiology, and drug discovery. Researchers use flow cytometry to identify and quantify different immune cell populations, study cell proliferation, assess cell viability, and analyze cell signaling pathways. Manufacturers like BD Biosciences (e.g., FACSCanto, FACSAria), Beckman Coulter (e.g., CytoFLEX, Gallios), and Thermo Fisher Scientific (e.g., Attune NxT) offer a range of flow cytometer models. When seeking cost-effective solutions, refurbished flow cytometers offer a viable alternative to purchasing new equipment. At American Laboratory Trading (ALT), we offer a comprehensive selection of refurbished flow cytometers from leading manufacturers. Our experienced in-house engineers meticulously refurbish and test each instrument to meet original equipment manufacturer (OEM) specifications, ensuring reliable performance. Every refurbished flow cytometer from ALT comes with a 90-day warranty, upgradable to one year, and includes lifetime support for your peace of mind. We also offer a price match guarantee and flexible payment options through ALT Capital to further support your research endeavors. Our large inventory and white-glove service options help streamline the integration of your new equipment into your lab. We understand the critical role of reliable lab equipment in scientific advancements and are committed to providing high-quality instruments while helping you manage your budget effectively. - Less