Your Trusted Source for Used Lab Equipment
Your Trusted Source for Used Lab Equipment
The Design-Build-Test-Learn (DBTL) cycle embodies the essence of synthetic biology, guiding researchers on a journey of discovery and innovation. By seamlessly integrating design principles, molecular engineering, empirical testing, and iterative learning, this process empowers scientists to engineer biological systems with unprecedented functionality.
Open an Interactive/Clickable Copy: Synthetic Biology Workflow
DESIGN. The process starts with clearly defining the problem to be solved or the goal to be achieved including identifying the desired biological function or trait to be engineered. Researchers design the DNA sequences needed to encode the desired biological functions including designing new genes, modifying existing genes, or assembling genetic circuits.
BUILD. Researchers synthesize DNA or isolate and purify genomic DNA which is then assembled into larger DNA constructs or vectors. This may involve techniques such as polymerase chain reaction (PCR), Gibson assembly, or Golden Gate assembly. The assembled DNA is then cloned into a suitable host organism, such as bacteria or yeast. The cloned DNA constructs are introduced into the host organism through a process called transformation (for bacteria) or transfection (for eukaryotic cells). This step is crucial for getting the engineered DNA into the cells where it will be expressed.
TEST. In vitro characterization techniques allow researchers to observe how the engineered system behaves in living cells. By conducting a battery of assays and experiments, scientists assess the system's function, performance, and robustness. This phase provides valuable insights into the system's behavior under various conditions.
LEARN. DNA sequences and experimental conditions may need to be optimized to improve the performance of the engineered system. These insights inform iterative refinements to the design, guiding subsequent cycles of the DBTL process. Each iteration brings scientists closer to optimizing the biological system for its intended application.
© Heritage ALT LLC All Rights Reserved