The Octet® RH16 Biolayer Interferometry (BLI) System is a protein analyzer offering unparalleled precision and efficiency in analyzing molecular interactions without the need for labels or secondary reagents. Engineered with cutting-edge BLI (Bio-Layer Interferometry) technology, this system enables real-time, label-free detection of biomolecular interactions, delivering swift and accurate results for researchers and scientists across various fields.

With its capacity to simultaneously analyze up to 16 samples, the Octet® RH16 streamlines experimental workflows, significantly reducing assay time and increasing throughput without compromising on data quality. Its high sensitivity and wide dynamic range allow for the measurement of interactions across various biomolecular targets, including proteins, antibodies, peptides, and small molecules.

This versatile system offers customizable assay formats, accommodating diverse experimental needs and ensuring adaptability to different research environments. Its user-friendly interface and intuitive software empower researchers to effortlessly design experiments, monitor interactions in real-time, and analyze data with precision.

Specifications:

Technology: Bio-Layer Interferometry (BLI) based on fiber optic biosensors
Sample volume: 40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered
Data presentation: Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts
Automation: Robot compatible microplate and biosensor tray loading
Orbital flow capacity: Static or 100–1500 rpm
Analysis temperature: Ambient + 4°C to 40°C, in 1°C increments
Association rate constant (ka): 101 to 107 M-1 s-1
Dissociation rate constant (kd): 10-6 to 10-1 s-1
Affinity constant (KD): 1 mM to 10 pM
Baseline noise (RMS): ≤ 4 pm (RMS)
Baseline drift: < 0.1 nm/hour
Quantitation range: 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm
Quantitation precision: CV <10%
Weight: 150 lb (68.2 Kg)
Dimensions: 31.5" W x 31.5" D x 30.1" H inches (80 x 80 x 77 cm)
Power: 120V, 60Hz
Sample type: Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates
Sample plate: Standard, 96-well and 384-well, black, tilted bottom microplates
Molecular weight detection: >150 Da (8–16 biosensors)