Category: HPLC, FPLC, & LC/MS | Subcategory: UPLC System

Waters NanoAcquity UPLC System Including: Binary Solvent Manager, Sample Manager With Integrated Heating and Trapping Module, Auxiliary Solvent Manager (Binary)

Waters NanoAcquity UPLC System Including: Binary Solvent Manager, Sample Manager With Integrated Heating and Trapping Module, Auxiliary Solvent Manager (Binary) Image

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Waters NanoAcquity UPLC System Including: Binary Solvent Manager, Sample Manager With Integrated Heating and Trapping Module, Auxiliary Solvent Manager (Binary) ImageWaters NanoAcquity UPLC System Including: Binary Solvent Manager, Sample Manager With Integrated Heating and Trapping Module, Auxiliary Solvent Manager (Binary) ImageWaters NanoAcquity UPLC System Including: Binary Solvent Manager, Sample Manager With Integrated Heating and Trapping Module, Auxiliary Solvent Manager (Binary) ImageWaters NanoAcquity UPLC System Including: Binary Solvent Manager, Sample Manager With Integrated Heating and Trapping Module, Auxiliary Solvent Manager (Binary) ImageWaters NanoAcquity UPLC System Including: Binary Solvent Manager, Sample Manager With Integrated Heating and Trapping Module, Auxiliary Solvent Manager (Binary) ImageWaters NanoAcquity UPLC System Including: Binary Solvent Manager, Sample Manager With Integrated Heating and Trapping Module, Auxiliary Solvent Manager (Binary) Image

The Waters nanoAcquity UPLC System has been designed for capillary to nano-scale separations to attain the highest sensitivity, resolution and reproducibility possible for biomarker discovery, and for proteomics applications for protein identification and characterization.

This system is particularly well-suited for applications in proteomics, metabolomics, and biopharmaceutical analysis, where high sensitivity and resolution are critical for the detection and quantification of low abundance biomolecules. With its cutting-edge technology and advanced features, the Waters nanoAcquity UPLC System is an essential tool for researchers and scientists working in the fields of life sciences, pharmaceuticals, and biotechnology.

This Waters nanoAcquity UPLC System includes:

  • Binary Solvent Manager
  • Sample Manager With Integrated Heating and Trapping Module
  • Auxiliary Solvent Manager (binary)
  • Computer pre-loaded with Software, Cart, Monitor, Keyboard, Mouse and Cables.

Specifications:

Solvent storage: Solvent tray accommodates up to four chromatographic solvents, two Sample Manager wash solvents and one Binary Solvent Manager wash solvent
Solvent conditioning:  Vacuum degassing (six channel): one channel per solvent and two channels for  Sample Manager wash solvents
Operating flow rate range: 200 nL/min to 100 μL/min without flow splitting
Compressibility compensation: Automatic and continuous
Effective system delay volume: <1 μL, from point of gradient mixing to analytical column; sample loop off-line
Plunger seal wash: Integral, active, programmable
Gradient profiles: 11 gradient curves [including linear, step (2), concave (4) and convex (4)]
Wet prime Automatic, user programmable
Maximum operating pressure:  10,000 psi
Primary wetted materials:  316 stainless steel, UHMWPE, sapphire, ruby, FEP, PTFE, ETFE, diamond-like coating,  PEEK and PEEK alloys, titanium alloys, alumina ceramic, fused silica
Gradient precision: <0.25 min standard deviation for retained peptides, n=6

CONDITIONS
Flow rate:  400 nL/min
Sample: MassPREP™ Enolase Digestion Standard (p/n 186002325)
Injection mode: Partial loop in 5 μL loop (injection volume 1.0 μL)
Column: 100 μm i.d. X 100 mm, Waters BEH 130, 1.7 μm column
Column temp.: 35°C
Eluent A: Water/0.1% formic acid
Eluent B: Acetonitrile/0.1% formic acid
Gradient: Linear from 5 to 50% B over 30 minutes 
Detection: UV at 214 nm 

SAMPLE MANAGER
Sample plates, 2: 96 and 384 microtiter plates; 48 position 2 mL vial plates; 48 position 0.65 mL micro-centrifuge tube plates; 24 position 1.5 mL micro-centrifuge tube plates 
Maximum sample capacity: 768 in two 384-well plates 
Number of sample injections: 1 to 99 injections per sample
Injection volume range: 0.1 μL to 50 μL, in 0.1 µL increments, partial or full loop mode, 5-µL loop is standard
Sample delivery precision: <0.3% RSD, full loop, 3x overfill, 5 to 50 µL (default wash/purge conditions, degassed methanol:water, 60:40 pre-mix, 1 mL/min, six replicates, propylparaben mix, 254 nm)
Injector linearity: >0.999 coefficient of deviation (from 2 to 10 µL, partial loop mode using 20-µL loop with 2 µL air gaps – no conditions cited)
Sample temperature control: 4 to 40 °C, programmable in 0.1 °C increments (with ambient temperature of 20 °C)
Sample probe: XYZZ based needle-in-needle design
Minimum sample required: 5 µL, using maximum recovery 2 mL vials
Wash solvents: Two degassed: strong solvent and sample compatible solvent, programmable to suit application, made available from the Binary Solvent Manager
Sample carryover: <0.005% or <2.0 nL, whichever is greater (with dual wash) 

HEATING AND TRAPPING MODULE
Column heater: Accommodates one column, up to 250 mm length x 75 μm, pivots out for use with mass spectrometer
Column temperature control: 5 °C above ambient to 65 °C, 0.1 °C increments

AUXILIARY SOLVENT MANAGER
Flow rate range: 200 nL/min to 50 µL/min
Column trapping pump: Choice of two eluents
Flow rate range: 1 μL/min to 100 μL/min
Acoustic noise: <65 dBA
Operating temperature range: 15 to 28 °C (58 to 82 °F)
Operating humidity range: 20% to 80%, noncondensing
Dimensions: 13.5" W x 28" D x 36.3" H (34.3 x 71.1 x 92.2 cm)
Power: 90 to 264V, 60Hz

 

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